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KMID : 0357419960260020251
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Korean journal of Virology
1996 Volume.26 No. 2 p.251 ~ p.258
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Sequence and Phylogenetic Analysis of V3 Region of Human Immunodeficiency Virus Type 1 Strains Isolated From Korean Patients
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김영봉
조영걸/이희정/정구헌/김정우/김유겸/양재명
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Abstract
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The V3 loop, a hypervariable domain of envelope glycoprotein, has an essential role in viral infectivity and has a major epitope for type-specific neutralizing antibody. In order to investigate genetic diversity of V3 region of gp120 of human
immunodeficiency virus type 1(HIV-1) isolated from Korean patients, DNA sequences encoding the C2 to V3 region were amplified by nested polymerase chain reaction (PCR) from uncultured peripheral blood mononuclear cells obtained from 15 HIV-1
seropositive patients and nucleotide sequences were determined. All nucleotide sequences from fifteen patients were compared with 8 distincitve subtypes (A-H) and another subtype O. Phylogenetic analysis was carried out with PHYLIP ver 3.5
(Dnapars)
program. Of the 15 isolates. 14 HIV-1 subjects were clustered with subtype B, while one was clustered with subtype C. Intra-subtype B distance at the nucleotide and deduced amino acid level were maximum 17.7% and 37.3%, respectively.
Intra-patient
distance at the nucleotide and deduced amino acid level were maximum 7.3% and 17.8%, respectively. Analysis of the nucleotide sequences revealed that Korean types have relatively well conserved sequences. These flndings could be useful for
assessing the
source of infection and developing an AIDS vaccine.
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KEYWORD
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